Title: Identification of functional SNP of FASN related to lipid metabolism in Korean cattle

Abstract:

Identification of functional SNP of FASN related to lipid metabolism in Korean cattle

In Korea, intramuscular fat (IMF) or marbling in a cross-section of longissimus dorsi muscle area (LM) tissue of cattle is an important component of carcass traits that have an impact on grading of beef quality and determining the meat price. The deposition of fat is mainly determined by lipid metabolism, which indicates the balance between fat deposition and fat removal in the LM. Also, the lipid metabolic gene may contribute to fat deposition. Among these genes, fatty acid synthase (FASN) is a multifunctional enzyme in fatty acid biosynthesis. This complex catalyzes the synthesis of the saturated fatty acids using acetyl-CoA, malonyl CoA and NADPH. FASN is a homodimer of 250 kDa subunits and contains seven catalysis activities and the acyl carrier protein (ACP). Many studies had reported that g.841G, g.16024A, g.16039T and g.17924G SNPs had a significant impact on marbling score in Korean cattle and Japanese black cattle population. These SNPs is a non-synonymous SNP to change amino acid and is in transcription factor binding site and beta-ketoacyl reductase, thioesterase region. The aim of this study was to investigate the relationship between these SNPs of FASN gene and marbling score of longissimus dorsi muscular area in Korean cattle. Among these SNPs, the g.841G and g.17924G SNP had a significant impact on marbling score (P<0.001). Therefore, our result suggests that, g.841G and g.17924G SNP will be a causal mutation in fatty acid biosynthesis. But we need further research for validating causal mutation as a site directed mutagenesis.

Biography:

Byung Yoon Choi has his expertise in evaluation and passion in gene editing and plant synthetic biology for producing high-quality meat. He is currently working with genes. He is expertise within the synthesis and investigation of physical and chemical properties of Nucleic Acids and their analogs and derivatives. One of other areas of his interests are: determination of thermodynamic parameters of DNA complexes formation. Investigation of spatial (2D and 3D) organization of DNA and DNA-protein complexes using physical methods.