Title: Phytochemical and pharmacological screening of leaves of barleria lupulina and ADME/T properties analysis of its isolated compounds.

Abstract:

The present study was undertaken to demonstrate potential ADME/T properties analysis and to evaluate antioxidant, anthelmintic and membrane stabilizing activities of the ethanol extract of Barleria lupulina leaves in different experimental models established in vitro. The dried leaves of B. lupulina were subjected to extraction using ethanol. Phytochemical screening was carried out using different chemical group tests. ADME/T Properties analysis test was carried out using Swiss ADME and online based tools respectively on nineteen isolated compounds. To investigate antioxidant property, three potential tests namely DPPH free radical scavenging study, Total Phenolic Content which was analyzed by the Folin-Ciocalteu’s colorimetric method using Gallic acid as standard and Total Flavonoid Content by using Quercetin as a standard. The Anthelmintic test was conducted on parasite Paramphistomum using four different concentrations (25, 50, 100, 200 mg/ml) of the extract and albendazole as standard drug (concentration 15mg/ml). To estimate the membrane stabilizing activity, both heat and unheat solution induced haemolysis techniques were used and Diclofenac sodium also used as standard drug.Phytochemical group tests indicate the presence of flavonoids, tannins, glycoside, steroids, carbohydrate and quinone. In ADME/T properties analysis, potential drug candidates were identified from the isolated compounds of Barleria lupulina. This plant has 19 compounds which accomplish Lipinski’s rule of five. In the quantitative assay, the extract showed antioxidant activity in DPPH free radical scavenging assay, the IC50 value was found to be 98.93μg/ml, Total Phenolic Content (TPC) indicated that 1mg of leaf extract contains 153.32 μg with GAE and also Total Flavonoid Content (TFC) was found to be 32.26μg with quercetin equivalence. The ethanolic extract showed potent anthelmintic activity at the highest concentration as it required less time for paralysis and death compared to the standard drug albendazole. The extract exhibited good membrane stabilizing activity inhibiting both heat and unheat solutions in comparison to inhibition by standard diclofenac sodium.The results presented that the ethanol extract of the plant has potential antioxidant, anthelmintic and membrane stabilizing activity based up on the experimental conditions tested. This study also expressed that the plant contains some potential chemical compounds which satisfy Lipinski’s rule of five (RO5) and might act as potential drug candidates.

Biography:

Niaj Morshed is from khwaja yunus ali university at Bangladesh. His research interest is phytochemical and pharmacological screening of leaves of barleria lupulina and adme/t properties analysis of its isolated compounds.