Title: PROBIOTIC PROPERTIES ANALYSIS OF ISOLATED LACTIC ACID BACTERIA FROM MILK

Abstract:

Lactic acid bacteria (LAB) are essential ingredients in probiotic foods, intestinal microflora, and dairy products that are capable of coping up with harsh gastrointestinal tract conditions and are available in a variety of environments. The objective of this study is to evaluate the probiotic property of LAB isolated from bovine milk. Milk samples were collected from local dairy farms. Samples were obtained using sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization. Preliminary Physiological and biochemical identification of LAB isolates was conducted by growing on MRS agar after ten-fold serial dilution. Seven of the best isolates were selected for the evaluation of the probiotic property. The LAB isolates were checked for resistance to antibiotics and their antimicrobial activity by disc diffusion assay and agar well diffusion assay respectively. Bile salt hydrolase activity of isolates were studied by growing isolates in BSH medium with bile salt. Cell surface property of isolates was assayed by studying their autoaggregation and coaggregation percentage with S. aerues. All isolates were found BSH positive. In addition, BCM2 and BGM1 were susceptible to all antibiotic disks except BBM1 which was resistant to all antibiotic disks. BCM1 and BGM1 had the highest autoaggregation and coaggregation potential respectively. Since all LAB isolates showed gastro intestinal tolerance and good cell surface property they could be considered as good potential probiotic candidates for treatment and probiotic starter culture preparation

Biography:

Alazar Essayas Ph.D Scholar, Department Science and Technology ,Sharda University Greater Noida(U.P). Lecturer ,Wollo University,Dessie ,Ethiopia

Title: Investigation of different agro-industrial wastes as bio-economical substrates to produce fungal laccase

Abstract:

Lignocellulosic biomass is made up of three main fractions: cellulose and hemicellulose and lignin. It is considered mainly as waste, but chiefly, as the most abundant renewable carbon source on the planet. Therefore,recently it has received a lot of attention as a potential source of bioenergy and bio-based chemicals. Theobjective of this study is to develop a valorization white biotechnology approach which allows both producingenzymes members of the lignocellulolytic degradation machinery, mainly laccases, and treating all the fractionsof lignocellulosic biomass at a time. Hence, in this work five different lignocellulosic sources from Algeria werechosen according to their industrial exploitability properties, their abundance and their availability, which werealfa stems (Stipa tenacissima) (ALF), olive pomace (Olea europaea) (OLV), tomato residues (Solanumlycopersicum) (TMT), dry palm leaves (Phoenix dactylifera) (PLM) and pinecones (Pinus halepensis) (PIN).Among 12 laccase positive strains, only one fungal strain Trametes versicolor was able to produce laccase onthese complex media by submerged fermentation. Indeed, variability in the enzyme secretion according to thetype of the substrate used, has been perceived, thus, the most suitable wastes for producing laccase were TMT,OLV, and PLM. The results reveal that the optimum of the laccase activity was obtained on the TMT medium55.6 U/L against 26,27U/L for OLV and 10.56U/L for PLM. The preliminary results of this research work, showthat tomato waste can be optimized to be used as a potential biological resource to produce laccases that oxidizeboth phenolic and non-phenolic compounds bound to lignin as well as highly recalcitrant environmentalpollutants, contributing at the same time to the biovalorisation of lignocellulosic biomass, which makes themvery useful for their application to several biotechnological processes.

Biography:

Applied Microbiology Lab, Ecole Interfacultaire de Bioingénieurs (EIB), Université Libre de Bruxelles, Belgique.