Title: The lack of fam83h mediated reduction of wnt/?-catenin signaling pathway and expression levels of dental mineralization genes

Abstract:

Background: FAM83H has been identified as an essential gene for dental enamel formation and may be related to Wnt/β-catenin. Methods: levels of Fam20a, Dspp, Dmp1, Enam, Ambn, Sppl2a, Mmp20, Fgf10, and the mediators of Wnt/βcatenin pathway were measured in the dental root of both Fam83h-KnockOut and wild-type mice by using QPCR at 5,11 and 18 days after birth. The expression of Fgf10 and the mediators of Wnt/β-catenin were also evaluated in the skin of KnockOut and wild-type mice by using Q-PCR and also, The histology of hair follicles was compared. the Fam83h-KnockOut mice recruited in this study confirmed by Sanger sequencing and western blot analysis. Results: Our results showed that Ambn, Mmp20, Dspp, and Fgf10 significantly reduced in Fam83h-KnockOut mice in the dental root, associated with marked reduction of CK1a, CK1e, and β-catenin expression in Fam83hKnockOut mice in the dental root. The Fgf10, CK1a, CK1e, and β-catenin were significantly decreased in the skin of Fam83h-KnockOut mice. In the absence of Fam83h, the accumulation of unemployed CK1a is expected to elevate the β-catenin destruction complex. The reduction of CK1e may also decrease the signaling of Dvl-1, leading to the suppression of the Wnt/β-catenin pathway. Simultaneous reduction of both mineralization genes and Wnt/β-catenin pathway due to the absence of Fam83h has a potential to be related to the deficiency of dental formation and mineralization. Further, concurrent reduction of Fgf10 gene expression and Wnt/β-catenin pathway may also affect the maturation of hair follicles as confirmed by histological examination. Conclusion: it seems that in the lack of Fam83h, dental mineralization is induced by simultaneous decrease of Wnt / β-catenin mediators and the mineralization-related genes, suggesting to act in a cumulative effect manner and probably behave as a multi-factorial trait.

Biography:

Sheikh Sabrina, an Assoc. Prof. In Azerbaijan Medical University, 1966. In 1988, graduated Az. Med.University pharmaceutical faculty. In 1988-1993 worked on the theme "Atherosclerosis correction by triterpenoids". Ph.D. in biological science. Nationality: Azerbaijani, Is a co-author of 2 books: "Biochemical laboratory classes" and " "Biochemistry laboratory manual and theory and methods", an editor of book "Pathological and clinical biochemistry" An author of teaching aids: "Biochemical mechanism of biotransformation and elimination of xenobiotics" An author of teaching aids: " Biochemical aspects of aging", "Some points in biochemistry and pathobiochemistry of the cell", "Role of apoptosis in the pathogenesis of certain diseases", "Biochemical aspects of ethanol action and metabolism", An author of annual biochemistry test-books for students of treatment, dental, pharmaceutical faculties, and test-book in clinical biochemistry. An expert in "State Commission for post-graduates"